The PDMS SlipChip is a novel and revolutionary microfluidic device. This represents a huge leap forward for biomedical research as it allows for much safer cell studies that allow for effective gradient generation. With our patented method for using low-viscosity silicone oil—specifically a 50 cSt silicone formula—our researchers created this inventive new tool. The advance published in the journal Micromachines promises to revolutionize drug development and advanced cell research.
The PDMS SlipChip goes directly to the heart of the problems that plague traditional cell studies. It stops channel clogging and decreases the likelihood of cell death. Using a two-step curing method, researchers maximized the device’s performance. To detect these cells, they heated the top layer of polydimethylsiloxane (PDMS) to 80°C. At the same time, they kept the bottom layer at 60°C, which helped fuse the two layers together and improve the overall stiffness of the material.
Aside from scalability, the most exciting aspect of the PDMS SlipChip is its ability to produce a stepwise concentration gradient. This major new capability opens many experimental applications that are absolutely game changing. The device proved effective in killing human osteosarcoma cells, a type of cancer cell. Remarkably, 95% of these cells survived in vivo during the transforming experiments using 50 cSt silicone oil. This demonstrates viability rates that compete with those achieved on traditional cell culture plates.
Also, the PDMS SlipChip previously shown to be able to produce a highly controlled gradient of Rhodamine 6G dye successfully validated its functions again. The improvement was dramatic when silicone oil of 120 mPa·s as lubricant was added during the sintering process. It provided an extra margin of performance consistency, especially at extremes of pneumatic pressure.
The PDMS SlipChip’s STM fabrication method and innately high-throughput design have researchers in the field of biomedical science excited for its potential. Its advanced functionality will allow for much more sophisticated studies exploring both normal cellular function, as well as pathologies. Moreover, it would streamline the drug testing process. This cutting-edge advancement holds tremendous promise to revolutionize the way scientists study cell-to-cell interactions and drug responses in highly controlled environments.